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  • Title: Alkaline endonuclease(s) activity in the thymus and spleen of normal and irradiated mice.
    Author: Suciu D, Uray Z.
    Journal: Int J Radiat Biol Relat Stud Phys Chem Med; 1977 Dec; 32(6):553-60. PubMed ID: 22504.
    Abstract:
    Evidence has been found on an alkaline endonuclease activity in the cytoplasmic and nuclear fraction isolated from the thymus and spleen mice. The chromatin-associated endonuclease activity was identified only in the spleen. The enzyme(s) was active on both single- and double-stranded DNA, but the reaction was faster if single-stranded DNA was used as a substrate. Maximum activity was found in the pH range of 7.9 to 8.1 in the presence of 10 mM Mg2+ and 1 mM Ca2+. The enzyme(s) splits DNA, yielding 3'-hydroxyl terminated polynucleotides. It is suggested that this alkaline endonuclease(s) is responsible for the formation of deoxyribopolynucleotides in the thymus and spleen of irradiated mice.
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