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  • Title: Examination of the mechanism of Na+/phosphate cotransport. Use of fluorophosphate and the nature of cotransporter functional asymmetry.
    Author: Peerce BE, Kiesling C.
    Journal: Miner Electrolyte Metab; 1990; 16(2-3):125-9. PubMed ID: 2250618.
    Abstract:
    The effect of substrates and mono- and di-fluorophosphates on Na+/phosphate cotransport by intestinal SDS-BBM vesicles was examined. Internal Na+ inhibited Na+/phosphate cotransport and this inhibition was relieved by internal phosphate. Inhibition by internal Na+ displayed Michaelis-Menten kinetics in contrast to the sigmoidal behavior for Na+ activation of phosphate uptake. Difluorophosphate but not monofluorophosphate inhibited Na+/phosphate cotransport in the external medium. In contrast, in the absence of internal Na+ monofluorophosphate but not difluorophosphate inhibited phosphate uptake. These results suggest that the intestinal Na+/phosphate cotransporter is functionally asymmetric in its substrate specificity.
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