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  • Title: [Study on tissue culture and plant regeneration of the stem-tips and buds of Periploca forrestii].
    Author: Liu ZF, Gao J, Niu YH, Shi L, Chen TT.
    Journal: Zhong Yao Cai; 2011 Nov; 34(11):1656-60. PubMed ID: 22506382.
    Abstract:
    OBJECTIVE: To establish the rapid propagation systems of the stem-tips and buds of Periploca forrestii. METHODS: Inserted the stem-tips and buds of Periploca forrestii into MS medium with different concentrations of 6-BA, NAA and 2.4-D and induced them growing into complete plants. RESULTS AND CONCLUSION: The optimal culture medium for bud induction is MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L and the bud induction rate can reach 86.29%. The optimal culture medium for stem-tips induction is MS +6-BA 2.0 mg/L + NAA 0.5 mg/L and the bud induction rate can reach 86.29%. The optimal culture medium for bud multiplication is MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L and the multiplication coefficient can reach 2.10. The best rooting medium is 1/2 MS + IBA 0.5 mg/L and the rooting rate is 53.33%.
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