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  • Title: [Characteristics of nonenzymatic binding of oligoribonucleotides-- analogs of mRNA and Phe-tRNA Phe with 80S ribosomes from human placenta].
    Author: Graĭfer DM, Zenkova MA, Karpova GG, Malygin AA, Matasova NB.
    Journal: Mol Biol (Mosk); 1990; 24(4):1076-83. PubMed ID: 2250674.
    Abstract:
    Binding of labelled oligouridylates--mRNA analogs--to human placenta 80S ribosomes in the presence of Phe-tRNAPhe has been studied. The single site for (pU)n (n = 6, 9, 13) binding on the ribosome was found; association constants for their tRNA-dependent binding were evaluated. In the presence of oligouridylates as templates [14C]Phe-tRNAPhe was found to be able to bind simultaneously at acceptor and donor ribosomal sites which resulted in diphenylalanine formation. The observed maximum Phe-tRNAPhe binding level was considerably lower than for the corresponding oligouridylate binding; the longer oligouridylate the higher Phe-tRNAPhe maximum binding level. To explain the results obtained we have proposed that (i) (Phe)2-tRNA produced from transpeptidation dissociates from the ribosomal A site to a significant extent and (ii) when oligouridylate length increases its interaction with 3'-side of mRNA binding center results in allosteric stabilization of the complex of peptidyl-tRNA with the ribosome at A site.
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