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  • Title: Improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles.
    Author: Mukhopadhyay A, Dasgupta AK, Chattopadhyay D, Chakrabarti K.
    Journal: Bioresour Technol; 2012 Jul; 116():348-54. PubMed ID: 22541951.
    Abstract:
    The activity and half-life of pectate lyase (PL) from Bacillus megaterium were nine- and 60-fold, respectively, higher at 90 °C in the presence of hydroxyapatite nanoparticles (NP-PLs) than in the presence of 1mM CaCl(2). Thermodynamic analysis of the nanoparticle-induced stability revealed an enhanced entropy-enthalpy compensation by the NP-PLs since a reciprocal linearity of the enthalpy-entropy change to 90 °C was observed. Without nanoparticles, the linearity range was 70 °C. Such compensation reflected the maintenance of the native structure of proteins. The remarkable enhancement of activity and stability of the NP-PL system at high temperatures may be utilized commercially e.g. in the food industry or the processing of natural fibers that may require a thermotolerant enzyme.
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