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  • Title: Cistanche deserticola extract increases bone formation in osteoblasts.
    Author: Li TM, Huang HC, Su CM, Ho TY, Wu CM, Chen WC, Fong YC, Tang CH.
    Journal: J Pharm Pharmacol; 2012 Jun; 64(6):897-907. PubMed ID: 22571269.
    Abstract:
    OBJECTIVES: We investigated the effect of Cistanche deserticola Ma. (CD) on bone formation by cultured osteoblasts. METHODS: The mineralized nodule formation assay was used to examine the in-vitro effects of CD on bone formation. Alkaline phosphatase (ALP), bone morphogenetic proteins (BMP)-2 and osteopontin (OPN) mRNA expression was analysed by quantitative real-time polymerase chain reaction. The mechanism of action of CD extract was investigated using Western blotting. The in-vivo anti-osteoporotic effect of CD extract was assessed in ovariectomized mice. KEY FINDINGS: CD extract had no effect on the proliferation, migration or wound healing of cultured osteoblasts, but increased ALP, BMP-2 and OPN mRNA and bone mineralization. Mitogen-activated protein kinase (MAPK) or nuclear factor (NF)-κB inhibitors reduced CD extract-induced bone formation and ALP, BMP-2 and OPN expression. However, CD extract did not affect osteoclastogenesis. In addition, CD extract prevented the bone loss induced by ovariectomy in vivo. CONCLUSIONS: CD may be a novel bone formation agent for the treatment of osteoporosis.
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