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  • Title: Electrophoresis of DNA fragments in gels from acrylamide-rich copolymers.
    Author: Christy KG, LaTart DB, Osterhoudt HW, Ponticello IS.
    Journal: Biotechniques; 1990 Oct; 9(4):480-5. PubMed ID: 2257146.
    Abstract:
    Two polyacrylamide-rich, non-toxic, gelable copolymers have been developed to facilitate the formation of user-cast electrophoresis gels. Gel formation is accomplished with dithiothreitol as the chemical cross-linking agent. The higher molecular weight copolymer is suitable for casting gels of copolymer concentration less than or equal to 8%. Gels of 3% concentration are excellent for resolving dsDNA fragments up to approximately 3000 base pairs. Because the cross-linking chemistry is not thwarted by the presence of urea, it is also possible to cast denaturing gels with these copolymers.
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