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  • Title: Fast self-assembly kinetics of quantum dots and a dendrimeric peptide ligand.
    Author: Wang J, Jiang P, Han Z, Qiu L, Wang C, Zheng B, Xia J.
    Journal: Langmuir; 2012 May 29; 28(21):7962-6. PubMed ID: 22582819.
    Abstract:
    Engineered peptide ligands with exceptionally high affinity for metal can self-assemble with nanoparticles in biological fluids. A high-affinity dendrimeric peptide ligand for CdSe-ZnS quantum dots (QDs) exhibited very fast association kinetics with QDs and reached equilibrium within 2 s. Here, we have combined a droplet-based microfluidic device with fluorescence detection based on Förster resonance energy transfer (FRET) to provide subsecond resolution in dissecting this fast self-assembly kinetics in solution. This work represents the first application of microfluidic devices to ligand-particle assembly for the measurement of fast assembly kinetics in solution.
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