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  • Title: Application of micro-scale liquid chromatography with fluorescence detection to the determination of thiols.
    Author: Ling BL, Dewaele C, Baeyens WR.
    Journal: J Chromatogr; 1990 Aug 29; 514(2):189-98. PubMed ID: 2258395.
    Abstract:
    Different thiol compounds of biological and pharmacological interest were separated on a packed reversed-phase fused-silica capillary column and determined with fluorescence detection. Conventional inexpensive liquid chromatographic equipment could be adapted for such purposes, providing a highly efficient analytical system. The different compounds were derivatized with the fluorogenic reagents SBD-F and ABD-F and chromatographed both isocratically and in the gradient mode in order to separate a series of thiols with widely varying polarities. Subsequently the derivatives were measured at lambda ex. = 380 nm and lambda em. = 510 nm. Application of the system to biological and pharmacological samples is suggested.
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