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  • Title: An LC method for the analysis of phosphatidylcholine hydrolysis products and its application to the monitoring of the acyl migration process.
    Author: Kiełbowicz G, Smuga D, Gładkowski W, Chojnacka A, Wawrzeńczyk C.
    Journal: Talanta; 2012 May 30; 94():22-9. PubMed ID: 22608409.
    Abstract:
    An assay for quantitative analysis of phosphatidylcholine (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and its hydrolysis products: 1-hydroxy-2-palmitoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine, sn-glycero-3-phosphocholine and palmitic acid using high-performance liquid chromatography with charge aerosol detector (CAD) was developed. The separation of the compounds of interest was achieved on a reversed-phase/hydrophilic interaction stationary phase with a mobile phase consisting of acetonitrile:methanol:10mM ammonium acetate solution. The method was applied to control the acyl migration process of LPC regioisomers in the most common solvents used in the synthesis or modification of PC.
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