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  • Title: A bioanalytical strategy utilizing dried blood spot sampling and LC-MS/MS in discovery toxicology studies.
    Author: Discenza L, Obermeier MT, Westhouse R, Olah TV, D'Arienzo CJ.
    Journal: Bioanalysis; 2012 May; 4(9):1057-64. PubMed ID: 22612686.
    Abstract:
    BACKGROUND & METHOD: The small sample volumes characteristic to dried blood spot (DBS) sampling enabled us to right-shift the linear dynamic range of an LC-MS/MS plasma assay tenfold and eliminate the need for extensive sample dilution in support of three discovery toxicology studies in which both plasma and DBS samples were collected. With the right-shifted DBS assay range, no DBS study samples required dilution, while all of the plasma samples were diluted 5-50-fold. RESULTS: DBS standard curves from 78-80,000 nM were linear, the performance of the curve and QC samples was within acceptable discovery-assay criteria and individual plasma and DBS data were comparable. Linear correlations of C(max) and AUC derived from DBS and plasma data resulted in R(2) > 0.9. CONCLUSION: This bioanalytical strategy represents a benefit to the bioanalyst that can expedite the return of data and minimize the potential for error and variability that can result from extensive dilutions of study samples.
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