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  • Title: In vitro culture of embryonic kidney rudiments and isolated ureteric buds.
    Author: Zhang X, Bush KT, Nigam SK.
    Journal: Methods Mol Biol; 2012; 886():13-21. PubMed ID: 22639247.
    Abstract:
    In vitro culture of embryonic kidney rudiments has been utilized to study a variety of cellular processes and developmental mechanisms. Here, we describe two-dimensional (2D) culture of embryonic kidney rudiments on Transwell filters and three-dimensional (3D) cultures in collagen gels in detail, and 3D culture of isolated ureteric bud (UB) in Matrigel with BSN-conditioned media.
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