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  • Title: [A feedback between membrane fluidity and transcription of the desB gene for the omega3 fatty acid desaturase in the cyanobacterium Synechocystis].
    Author: Mironov KS, Maksimov EG, Maksimov GV, Los' DA.
    Journal: Mol Biol (Mosk); 2012; 46(1):147-55. PubMed ID: 22642112.
    Abstract:
    Prokaryotic cells, including cyanobacteria, respond to a decrease in ambient temperature by activation of numerous cold shock genes. Low temperatures cause a decrease in membrane fluidity, which is maintained at some optimal level mainly by fatty acid (FA) desaturases. Here, temperature-dependent expression of the desB gene for the omega3-desaturase in Synechocystis, which synthesized polyunsaturated FAs, and in its mutant, desA-/desD-, which is defective in genes for delta12- and delta6-desaturases and is capable of synthesizing only monounsaturated FAs was studied. Low temperatures caused the increase in the amount of the desB mRNA in the wild-type cells with the maximum observed at 24 degrees C. In the double mutant desA-/desD- cells, the maximum amount of this mRNA was accumulated at 28-30 degrees C. Thus, our studies of the desB gene for the omega3-desaturase demonstrated that temperature-dependent expression of genes, which are responsible for the maintenance of the optimal membrane fluidity, depends on physical state of these membranes and is regulated by a feedback mode.
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