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  • Title: High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates.
    Author: Vorimore F, Cavanna N, Vicari N, Magnino S, Willems H, Rodolakis A, Siarkou VI, Laroucau K.
    Journal: J Microbiol Methods; 2012 Sep; 90(3):241-4. PubMed ID: 22652433.
    Abstract:
    We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
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