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  • Title: Cell surface-anchored fluorescent aptamer sensor enables imaging of chemical transmitter dynamics.
    Author: Tokunaga T, Namiki S, Yamada K, Imaishi T, Nonaka H, Hirose K, Sando S.
    Journal: J Am Chem Soc; 2012 Jun 13; 134(23):9561-4. PubMed ID: 22663380.
    Abstract:
    A fluorescent aptamer sensor was applied to the analysis of extracellular chemical transmitter dynamics. We utilized a tocopherol-labeled aptamer, which allowed the direct anchoring of the fluorescent aptamer on the cell surface while retaining its performance as a fluorescent sensor. The fast-responsive fluorescent DNA aptamer sensor, which targets adenine compounds, was anchored on the surface of brain astrocytes. Fluorescence imaging of the aptamer-anchored astrocytes enabled the real-time monitoring of release of adenine compounds as a gliotransmitter, which was synchronized with calcium wave propagation in neighboring cells.
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