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  • Title: Growth response of axenic Entamoeba histolytica to hydrogen, carbon dioxide, and oxygen.
    Author: Band RN, Cirrito H.
    Journal: J Protozool; 1979 May; 26(2):282-6. PubMed ID: 226695.
    Abstract:
    Entamoeba histolytica required CO2 for growth in axenic culture while growth was inhibited by H2. The organism was tolerant to 5% O2 in the gas phase and it was able to detoxify products of O2 reduction in the medium. The ameba did not require a negative oxidation-reduction potential for axenic growth. However, little or no free O2 was present in media exposed to 5% O2 in the gas phase. Growth was improved by adding yeast extract to the medium.
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