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  • Title: Impact of an extracellular polymeric substance (EPS) precoating on the initial adhesion of Burkholderia cepacia and Pseudomonas aeruginosa.
    Author: Hwang G, Kang S, El-Din MG, Liu Y.
    Journal: Biofouling; 2012; 28(6):525-38. PubMed ID: 22686692.
    Abstract:
    Extracellular polymeric substances (EPS) significantly influence bacterial adhesion to solid surfaces, but it is difficult to elucidate the role of EPS on bacterial adhesion due to their complexity and variability. In the present study, the effect of EPS on the initial adhesion of B. cepaciaepacia PC184 and P. aeruginosa PAO1 on glass slides with and without an EPS precoating was investigated under three ionic strength conditions. The surface roughness of EPS coated slides was evaluated by atomic force microscopy (AFM), and its effect on initial bacterial adhesion was found to be trivial. X-ray photoelectron spectroscopy (XPS) studies were performed to determine the elemental surface compositions of bacterial cells and substrata. The results showed that an EPS precoating hindered bacterial adhesion on solid surfaces, which was largely attributed to the presence of proteins in the EPS. This observation can be attributed to the increased steric repulsion at high ionic strength conditions. A steric model for polymer brushes that considers the combined influence of steric effects and DLVO interaction forces is shown to adequately describe bacterial adhesion behaviors.
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