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  • Title: Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgL.
    Author: Wolfram F, Arora K, Robinson H, Neculai AM, Yip P, Howell PL.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2012 May 01; 68(Pt 5):584-7. PubMed ID: 22691793.
    Abstract:
    The periplasmic alginate lyase AlgL is essential for the synthesis and export of the exopolysaccharide alginate in Pseudomonas sp. and also plays a role in its depolymerization. P. aeruginosa PAO1 AlgL has been overexpressed and purified and diffraction-quality crystals were grown using the hanging-drop vapour-diffusion method. The crystals grew as thin plates, with unit-cell parameters a = 56.4, b = 59.6, c = 102.1 Å, α = β = γ = 90°. The AlgL crystals exhibited the symmetry of space group P2(1)2(1)2(1) and diffracted to a minimum d-spacing of 1.64 Å. Based on the Matthews coefficient (V(M) = 2.20 Å(3) Da(-1)), one molecule is estimated to be present in the asymmetric unit.
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