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  • Title: Affinity purification of a 70K protein, a membrane protein relevant to sexual cell fusion in Dictyostelium discoideum.
    Author: Ishikawa T, Urushihara H, Habata Y, Yanagisawa K.
    Journal: Cell Differ Dev; 1990 Sep; 31(3):177-84. PubMed ID: 2271994.
    Abstract:
    Sexual cell fusion occurs between HM1 and NC4, heterothallic strains in Dictyostelium discoideum. A membrane component of HM1 cells with a molecular mass of 70 kDa (70K protein) has been shown to be implicated in cell fusion (Urushihara et al. (1988) Cell Differ. Dev. 25, 81-88). In the present study, 70K protein was partially purified using affinity Sepharose on which membrane proteins of NC4 cells were immobilized. Through this process, involvement of Ca2+ in the interaction of 70K protein with its receptor was suggested. Lectin staining of partially purified 70K protein indicated it to be a glycoprotein containing D-mannose and/or D-glucose residues.
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