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  • Title: Gas chromatographic determination of incurred dimetridazole residues in swine tissues.
    Author: Newkirk DR, Righter HF, Schenck FJ, Okrasinski JL, Barnes CJ.
    Journal: J Assoc Off Anal Chem; 1990; 73(5):702-4. PubMed ID: 2272993.
    Abstract:
    A gas chromatographic method for determination of 2-hydroxymethyl-1-methyl-5-nitroimidazole (DMZOH), the hydroxy metabolite of dimetridazole, in swime muscle has been developed. The method uses cleanup steps similar to those of an earlier polarographic method. The present method is capable of quantitating levels as low as 2 ppb and detecting less than 1 ppb. Recoveries from 30 control tissues spiked at 1, 2, or 4 ppb averaged 80.4%. Performance of the method in incurred tissue was documented and limited data on the depletion of the metabolite in muscle were generated. The muscle of swine given 150 ppm dimetridazole in feed for 14 days contained less than 1 ppb DMZOH at 12 h withdrawal time.
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