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  • Title: Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases--application to feed supplements.
    Author: Neubauer S, Rugova A, Chu DB, Drexler H, Ganner A, Sauer M, Mattanovich D, Hann S, Koellensperger G.
    Journal: Anal Bioanal Chem; 2012 Aug; 404(3):799-808. PubMed ID: 22752403.
    Abstract:
    In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100% aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L(-1) could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.
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