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  • Title: Separation and determination of trace environmental estrogen through molecularly imprinted solid phase extraction coupled to high performance liquid chromatography.
    Author: Jing Z, Chao XL, Li C, Xiang XZ, Guang QX, Ming SJ.
    Journal: J Environ Sci Health A Tox Hazard Subst Environ Eng; 2012; 47(12):1889-96. PubMed ID: 22755536.
    Abstract:
    In recent years, there has been an increasing demand on the determination of trace environmental estrogen. In this study, a new 17β-estradiol molecularly imprinted polymer was successfully synthesized on the surface of activated silica gel. This prepared molecularly imprinted polymer was characterized, and found to exhibit good recognition, high selectivity, and a rapid adsorption-desorption dynamic toward 17β-estradiol. Using the molecularly imprinted polymer as a sorbent, an effective method of solid phase extraction coupled to high performance liquid chromatography for the separation and determination of 17β-estradiol was developed. Under optimal conditions, the detection limit (S/N = 3) of 17β-estradiol was 12.0 ng L(-1). The enrichment factor was 198, and the linear range of the calibration graph was 0.05-300 mg L(-1). Blank water samples spiked with 17β-estradiol at three different levels were analyzed by the developed method to afford recoveries ranging from 82.7% to 103.0%. Moreover, this presented method was applied to the quantitative detection of 17β-estradiol in a polluted water sample with a level of 0.024 mg L(-1).
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