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  • Title: [Testing methods for seed quality of Cyathula officinalis].
    Author: Liu Q, Wu W, Luo H, Cai W, Chen Q.
    Journal: Zhongguo Zhong Yao Za Zhi; 2011 Jun; 36(11):1421-6. PubMed ID: 22779169.
    Abstract:
    OBJECTIVE: To study testing methods of seed quality, and provide a basis for establishing seed testing specification of Cyathula officinalis. METHOD: Referring to the Specifications for Agricultural Seed Testing, the optimal testing methods of seed quality of C. officinalis were screened. RESULT AND CONCLUSION: The testing method for C. officinalis seed quality has been initially established. At least 8 g seeds should be sampled and passed through 20-mesh sieve for purity analysis. The phenotypic observation and size measurement were used for authenticity testing. The seeds were inoculated directly on PDA medium, cultured 5 days on 28 degrees C for seed health testing. The weight of 1 000 seeds was determined by using the 500-seed method. The water content of the seeds was determined under the higher temperature (133 +/- 2) degrees C for 3 hours. The seeds were dipped into 0.1% TTC solution 3 hours for determining viability. The seeds were cultured on pleated paper at 25 degrees C for 2-9 days for germination testing.
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