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  • Title: [Identification of Junci Medulla using DNA barcoding technique].
    Author: Pang X, Song J, Chen S.
    Journal: Zhongguo Zhong Yao Za Zhi; 2012 Apr; 37(8):1097-9. PubMed ID: 22779357.
    Abstract:
    OBJECTIVE: To identify Junci Medulla using the ITS2 barcode. METHOD: The ITS2 regions of Juncus effuses and its closely related species were PCR amplified and sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. The Kimura 2-Parameter (K2P) distances were calculated using software MEGA 5.0. Identification analyses were performed using BLAST1, Nearest Distance, PWG Distance and neighbor-joining (NJ) methods. RESULT: The intra-specific genetic distances of J. effuses were ranged from 0 to 0.005, which were far lower than inter-specific genetic distances between J. effuses and its closely related species (0.215-0.614). All the four methods showed that ITS2 could discriminate J. effuses from its closely related species correctly. CONCLUSION: The ITS2 region is an efficient barcode for authentication of Junci Medulla, and our study further confirmed the ability of ITS2 to identify traditional Chinese medicinal materials.
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