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  • Title: Labeling and imaging mesenchymal stem cells with quantum dots.
    Author: Collins MC, Gunst PR, Cascio WE, Kypson AP, Muller-Borer BJ.
    Journal: Methods Mol Biol; 2012; 906():199-210. PubMed ID: 22791434.
    Abstract:
    Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, -cartilage, adipose, and muscle cells. Adult derived MSCs are being actively investigated because of their potential to be utilized for therapeutic cell-based transplantation. Methods to track MSCs in vivo are -limited, preventing long-term functional studies of transplanted cells. Quantum Dots (QDs) offer an alternative to organic dyes and fluorescent proteins to label and track cells in vitro and in vivo. Nanoparticles are resistant to chemical and metabolic degradation, demonstrating long-term photostability. Here, we describe the technique to label MSCs with QDs and demonstrate intracellular QD distribution in the labeled MSCs with laser scanning confocal fluorescent microscopy.
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