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Title: Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells. Author: Adam T, Bouhidel K, Der C, Robert F, Najid A, Simon-Plas F, Leborgne-Castel N. Journal: FEBS Lett; 2012 Sep 21; 586(19):3293-8. PubMed ID: 22796492. Abstract: Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associated genes.[Abstract] [Full Text] [Related] [New Search]