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  • Title: Development of a novel chemiluminescence method for the determination of cefazolin sodium in injectable powder and human urine based on a luminol-Cu(III) complex reaction in alkaline medium.
    Author: Sun H, Wang J, Wang T.
    Journal: Luminescence; 2013; 28(4):592-6. PubMed ID: 22807137.
    Abstract:
    A novel chemiluminescence (CL) method was developed for the determination of cefazolin sodium based on the CL reaction between the [Cu(HIO6)2](5-) Cu(III) complex and luminol in alkaline solution. Results showed that CL emission of Cu(III) complex-luminol in alkaline medium was significantly different from that in acidic medium. A possible mechanism of the enhanced effect of cefazolin on CL emission of the [Cu(HIO6)2](5-)-luminol system was proposed. The effect of the reaction conditions on CL emissions was examined. Under optimized conditions, a good linear relationship was obtained between CL intensity and concentrations of cefazolin sodium in the range of 2.0 x 10(-8) to 2.0 x 10(-6) g/mL with a correlation coefficient of R(2) = 0.9978. The limit of detection was 4.58 x 10(-9) g/mL. The proposed method was applied for the determination of cefazolin sodium in real samples with recoveries of 82.0-109% with an RSD of 0.7-2.1%. The proposed method was successfully used for the determination of cefazolin sodium in injectable powder preparations and human urine with satisfactory results.
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