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  • Title: Assay of protein A in Staphylococcus hyicus subsp. hyicus by ELISA and immunoelectron microscopy.
    Author: Takeuchi S, Kobayashi Y, Mori Y.
    Journal: Vet Microbiol; 1990 Nov; 25(2-3):297-302. PubMed ID: 2281610.
    Abstract:
    The presence and quantity of protein A in Staphylococcus hyicus subsp. hyicus isolates were examined by an enzyme-linked immunosorbent assay (ELISA) and immunoelectron microscopy. Cell-bound protein A was demonstrated in 45 (94%) of 48 isolates from diseased pigs and in 113 (86%) of 132 isolates from healthy pigs by ELISA using peroxidase-conjugated rabbit antibody, but was not found in isolates from chickens and cows. Most of these swine isolates contained about 100 to 300 ng of cell-bound protein A/ml. Extracellular protein A was not detected in any isolates from pigs, chickens or cows. In the immunoelectron microscopy assay, swine isolates were labeled with goat anti-mouse IgG conjugated to colloidal gold particles, but chicken and cow isolates were not labeled.
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