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  • Title: Characterisation of poly(ADP-Rib) polymerase activity in nuclei from the slime mould Dictyostelium discoideum.
    Author: Rickwood D, Osman MS.
    Journal: Mol Cell Biochem; 1979 Oct 15; 27(2):79-84. PubMed ID: 228176.
    Abstract:
    Nuclei isolated from D. discoideum and incubated in vitro with 3H-NAD synthesise poly(ADP-Rib). The optimum incubation conditions for the poly(ADP-Rib) polymerase were determined. The Km of the enzyme is 18 microM NAD and it is inhibited by nicotinamide. Most of the poly(ADP-Rib) synthesised is attached to nuclear proteins.
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