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  • Title: Molecular characterization of Cryptosporidium in native beef calves in central Vietnam.
    Author: Nguyen ST, Fukuda Y, Tada C, Sato R, Duong B, Nguyen DT, Nakai Y.
    Journal: Parasitol Res; 2012 Oct; 111(4):1817-20. PubMed ID: 22828931.
    Abstract:
    The aims of this study were to investigate the prevalence of Cryptosporidium and to characterize the genotype distribution of Cryptosporidium isolates in native beef calves 2-6 months old in Dac Lac province, central Vietnam. The presence of Cryptosporidium oocysts was determined using the modified Ziehl-Neelsen staining method. The overall prevalence on the sample and herd levels were 18.9% (44/232) and 50% (20/40), respectively. Genotyping based on PCR and sequence analysis of the 18 S rRNA gene revealed occurrence of the two nonzoonotic species Cryptosporidium ryanae and Cryptosporidium bovis, with the former as a dominant species in the animals. The absence of the zoonotic species Cryptosporidium parvum in calves examined suggests that the native beef calves 2-6 months old in the study area are unlikely to contribute to human cryptosporidiosis transmission.
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