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  • Title: Optimization and characterization of a polysaccharide produced by Pseudomonas fluorescens WR-1 and its antioxidant activity.
    Author: Raza W, Yang W, Jun Y, Shakoor F, Huang Q, Shen Q.
    Journal: Carbohydr Polym; 2012 Oct 01; 90(2):921-9. PubMed ID: 22840021.
    Abstract:
    The extracellular polysaccharide produced by a newly isolated strain Pseudomonas fluorescens WR-1 was purified and characterized and its production was optimized using response surface methodology. The results showed that the strain WR-1 produced one kind of EPS that was composed of arabinose, glucose and uronic acid. The molecular weight of the EPS was determined to be 6.78 × 10(6)Da. The preferable culture conditions for EPS production were pH 7.0, temperature 28°C for 72 h with peptone and maltose as best N and C sources, respectively. The model predicted that the maximum EPS production (39.6 gL(-1)) was appeared with maltose 48.65 gL(-1), Mn(2+) 1118 μM and Zn(2+) 901 μM. The EPS also showed good H(2)O(2) scavenging activity while moderate free radical scavenging activity and reductive ability were determined. The EPS from WR-1 may be a new source of natural antioxidants with potential value for health, food and industry.
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