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Title: Validated determination of primulasaponins in primula root by a high-performance-thin-layer-chromatography densitometric approach. Author: Coran SA, Mulas S. Journal: J Pharm Biomed Anal; 2012 Nov; 70():647-51. PubMed ID: 22841554. Abstract: A novel HPTLC-densitometric method was developed for separation and quantitation of primulasaponin I and II in different matrices. HPTLC silica gel 60 F254(S), 20 cm × 10 cm, plates with ethyl acetate:water:formic acid (5:1:1 v/v) as the mobile phase were used. Densitometric determinations were performed in reflectance mode at 540 nm after derivatization with vanillin reagent. The method was validated giving rise to a dependable and high throughput procedure well suited to routine applications. Primulasaponins were quantified in the range of 150-450 ng with RSD of repeatability and intermediate precision between 0.8 and 1.4% and accuracy within the acceptance limits. The method was tested on commercial herbal medicinal preparations claiming to contain primula root extract.[Abstract] [Full Text] [Related] [New Search]