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  • Title: Purification and differential biological effects of ginger-derived substances on normal and tumor cell lines.
    Author: Almada da Silva J, Becceneri AB, Sanches Mutti H, Moreno Martin AC, Fernandes da Silva MF, Fernandes JB, Vieira PC, Cominetti MR.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2012 Aug 15; 903():157-62. PubMed ID: 22858304.
    Abstract:
    This study describes an optimization of [6]-, [8]- and [10]-gingerol isolation and purification in semi-preparative HPLC scale and their anti-proliferative activity. The gingerols purification was carried out in HPLC system using a Luna-C₁₈ and the best mobile phase evaluated was MeOH/H₂O (75:25, v/v). This new methodology for the gingerols isolation was very effective, since considerable amounts (in the range of milligrams) with a good purity degree (∼98%) were achieved in 30 min of chromatographic run. [6]-, [8]- and [10]-Gingerol purified by this methodology inhibited the proliferation of MDA-MB-231 tumor cell line with IC₅₀ of 666.2±134.6 μM, 135.6±22.6 μM and 12.1±0.3 μM, respectively. These substances also inhibited human fibroblasts (HF) cell proliferation, however in concentrations starting from 500 μM. In conclusion, our results demonstrate an optimization of gingerols isolation and their specific anti-proliferative activities against tumor cells, suggesting their use as important models for drug design in an attempt to develop new compounds with fewer side effects when compared to conventional chemotherapy.
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