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  • Title: [Simultaneous liquid chromatographic determination of plasma 3,4-dihydroxyphenylglycol and norepinephrine as indicators of sympathetic activity].
    Author: Okumura K, Ito T.
    Journal: Nihon Naibunpi Gakkai Zasshi; 1990 Nov 20; 66(11):1164-9. PubMed ID: 2286283.
    Abstract:
    A high performance liquid chromatographic method with electrochemical detection is described for simultaneously measuring the concentrations of the endogenous catecholamines and the deaminated catecholamine metabolite 3,4-dihydroxyphenyglycol (DHPG) in plasma. Plasma DHPG concentrations as determined by the present method were 734 +/- 77pg/ml after 30 min. of supine rest whereas plasma norepinephrine (NE) concentrations were 114 +/- 20pg/ml in 5 healthy subjects. The DHPG and NE concentrations increased to 891 +/- 64 and 266 +/- 36pg/ml, respectively, after 30 min. of upright position following 30 min. of supine rest. The simultaneous measurement of plasma DHPG and NE concentrations may provide a more comprehensive assessment of the sympathetic nervous system.
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