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  • Title: A rapid two-step purification of rat liver fetal thymidine kinase.
    Author: Baron G, Madec-Baron A, Sampérez S, Jouan P.
    Journal: Prep Biochem; 1990; 20(3-4):241-56. PubMed ID: 2287608.
    Abstract:
    The fetal isoenzyme of thymidine kinase was purified to apparent homogeneity from cytosols of rat fetuses liver. A two-step purification including anion exchange chromatography and affinity chromatography was developed. The purified enzyme appears as oligomeric with a relative molecular weight of 71 kDa. In denaturing media its molecular weight was 24 kDa, and its pHi 8.3.
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