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  • Title: [Study on HPLC fingerprint of Herba Lysimachiae].
    Author: Guo LL, Zhao D, Deng J.
    Journal: Zhong Yao Cai; 2012 Mar; 35(3):382-5. PubMed ID: 22876675.
    Abstract:
    OBJECTIVE: To establish the chromatographic fingerprint for Herba Lysimachiae by RP-HPLC. METHODS: The sample solutions were prepared by extracting material with boiling water,followed by extracting with BuOH from the aqueous extract and analyzed by RP-HPLC with a Phenomenex C18 column (4.6 mm x 150 mm, 5 microm), gradiently eluted with A (acetonitrile containing 0.5% of acetic acid)-B (water containing 0.5% of acetic acid) at 30 degrees C, and monitored with UV detector at 255 nm. The flow rate was 1.0 mL/min, and sample volume injected was 20 microL. The chromatograms of different batches of Herba Lysimachiae were compared by the software of Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004A). RESULTS: HPLC Fingerprint of Herba Lysimachiae was established with Rutin as the reference compound. 13 common peaks were selected as the fingerprint peaks of Herba Lysimachiae. CONCLUSION: The established HPLC fingerprint of Herba Lysimachiae has desirable precision, reproducibility, and can be applied to routine quality control of Herba Lysimachiae.
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