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  • Title: Correlation of migration behavior in free-flow zone electrophoresis and electrophoretic titration curve.
    Author: Nath S, Schütte H, Hustedt H, Deckwer WD.
    Journal: Electrophoresis; 1990 Aug; 11(8):612-6. PubMed ID: 2289459.
    Abstract:
    The correlation of electrophoretic migration behavior in free-flow zone electrophoresis (FFZE) and electrophoretic titration curve (ETC) has been explored. It is shown that the ETC of a protein or a mixture of proteins can be used to predict the fraction numbers at which those proteins elute in a preparative scale FFZE experiment. The ETC is a quick and effective way to choose optimal buffer conditions in FFZE. FFZE is employed to determine the isoelectric points (pI) of proteins whose pIs lie beyond the range of IEF 3-9 gels. It is found that separations in FFZE are governed by the net surface charge of the proteins.
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