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  • Title: An improved method for quantitative determination of urinary porphyrins by use of second-derivative spectroscopy.
    Author: van de Giessen AW, van Wijk EM.
    Journal: J Clin Chem Clin Biochem; 1990 Sep; 28(9):605-9. PubMed ID: 2290079.
    Abstract:
    An improved assay for quantification of urinary porphyrins by use of second-derivative spectroscopy is described. A new method for calculation of the porphyrin concentration is developed and the whole procedure is computerized. Acidified urine samples can be assayed within a few minutes by using this method. Precision and recoveries for both uro- and coproporphyrin are good. The method is presented as a very fast and accurate assay for the screening and quantification of urinary porphyrins.
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