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  • Title: Evaluation of micronuclei in tobacco users: A study in Punjabi population.
    Author: Bansal H, Sandhu VS, Bhandari R, Sharma D.
    Journal: Contemp Clin Dent; 2012 Apr; 3(2):184-7. PubMed ID: 22919220.
    Abstract:
    INTRODUCTION: The assessment of micronuclei in exfoliated cells is a promising tool for the study of epithelial carcinogens and can be used to detect chromosome breakage or mitotic interference, thought to be relevant to carcinogenesis. AIM: The present study aimed to detect micronuclei in exfoliated oral mucosal cells in individuals using various tobacco forms from the last 5 years. MATERIALS AND METHODS: A total of 75 healthy male subjects (25 smokeless tobacco users, 25 smokers, and 25 non-tobacco users) were selected for the study. Smears were obtained with moistened wooden spatula from buccal mucosa and fixed with 95% alcohol. All the cytologic smears were stained by Papanicolaou technique. From each slide, ~1000 cells were examined under the 400× magnification and where micronucleated (MN) cells were located, they were examined under the 1000× magnification. RESULT: MN cells were found to be significantly higher in smokeless tobacco users than in smokers and controls. CONCLUSION: A positive correlation is found between increased micronucleus frequency and tobacco-using habits. So micronucleus assay can be used as a biomarker of genotoxicity.
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