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  • Title: Metabolic response to exogenous ethanol in yeast: an in vivo statistical total correlation NMR spectroscopy approach.
    Author: Ricci M, Aggravi M, Bonechi C, Martini S, Aloisi AM, Rossi C.
    Journal: J Biosci; 2012 Sep; 37(4):749-55. PubMed ID: 22922199.
    Abstract:
    In vivo NMR spectroscopy, together with selectively 13C-labelled substrates and 'statistical total correlation spectroscopy' analysis (STOCSY), are valuable tools to collect and interpret the metabolic responses of a living organism to external stimuli. In this study, we applied this approach to evaluate the effects of increasing concentration of exogenous ethanol on the Saccharomyces cerevisiae fermentative metabolism. We show that the STOCSY analysis correctly identifies the different types of correlations among the enriched metabolites involved in the fermentation, and that these correlations are quite stable even in presence of a stressing factor such as the exogenous ethanol.
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