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  • Title: Development of microsatellite markers for Anadenanthera colubrina var. cebil (Fabaceae), a native tree from South America.
    Author: Barrandeguy ME, Prinz K, García MV, Finkeldey R.
    Journal: Am J Bot; 2012 Sep; 99(9):e372-4. PubMed ID: 22935360.
    Abstract:
    PREMISE OF THE STUDY: Microsatellite primers were developed in the native legume tree Anadenanthera colubrina var. cebil to study the genetic diversity and genetic structure in natural populations in Argentina. METHODS AND RESULTS: Nine microsatellite markers were identified using a genomic library enriched for tandemly repeated motifs, eight of which markers were polymorphic. The polymorphism of these markers was assessed by investigating 20 individuals for fragment polymorphism; three to 13 alleles were observed for each locus. Observed and expected heterozygosities ranged from 0.300 to 1.000 and from 0.463 to 0.900, respectively. CONCLUSIONS: These results confirm that these primers will be useful for investigating the genetic diversity and genetic structure of natural populations of A. colubrina var. cebil in future studies.
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