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  • Title: An effect of cell-culture passage on ultraviolet-enhanced viral reactivation by mammalian cells.
    Author: Moore SP, Coohill TP.
    Journal: Mutat Res; 1979 Oct; 62(3):417-23. PubMed ID: 229411.
    Abstract:
    The ability of ultraviolet-irradiated African green monkey kidney cells to reactivate UV-damaged herpes simplex virus was tested over a range of 57 passages (24-81). Several types of controls were employed to minimize daily variations. The results indicate that in this system cells demonstrate an increasing efficiency to reactivate UV-damaged virus until approx. passage 60, followed by a decrease in this ability beyond this passage. Chromosome counts at selected passages revealed no significant change in chromosome number as a function of passage. The mechanism(s) or factor(s) causing this effect are unknown.
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