These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Ultrasensitive and selective homogeneous sandwich immunoassay detection by Surface Enhanced Raman Scattering (SERS).
    Author: Pekdemir ME, Ertürkan D, Külah H, Boyacı IH, Ozgen C, Tamer U.
    Journal: Analyst; 2012 Oct 21; 137(20):4834-40. PubMed ID: 22943047.
    Abstract:
    In this report, a simple and highly selective homogeneous sandwich immunoassay was developed for ultrasensitive detection of Staphylococcal Enterotoxin B (SEB) using Surface Enhanced Raman Scattering (SERS). The assay uses polyclonal-antibody functionalized magnetic gold nanorod particles as capture probes for SEB, which can be collected via a simple magnet. After separating SEB from the sample matrix, they are sandwiched by using binding-specific antibody-antigen pairs with the help of gold nanorod particles. Gold nanorod particles are bifunctional by design and contain self-assembled monolayers (SAMs) of a SERS tag molecule (5,5-dithiobis (2-nitrobenzoic acid), DTNB) and carboxylic functionalities of DTNB for coupling with a suitable antibody. The correlation between the SEB concentration and SERS signal was found to be linear within the range of 3 fM to 0.3 μM. The limit of detection for the assay was determined to be 768 aM (ca., 9250 SEB molecules per 20 μL sample volume). The gold heterogeneous assay system for SEB detection was also compared with the same SERS probes and gold-coated surfaces as capture substrates. The developed method was further evaluated for detecting SEB in artificially contaminated milk. Finally, the method was used for investigating the SEB specificity on bovine serum albumin (BSA) and avidin.
    [Abstract] [Full Text] [Related] [New Search]