These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Regulation of the murine alpha A-crystallin promoter in transgenic mice.
    Author: Wawrousek EF, Chepelinsky AB, McDermott JB, Piatigorsky J.
    Journal: Dev Biol; 1990 Jan; 137(1):68-76. PubMed ID: 2295367.
    Abstract:
    To identify sequences necessary for lens-specific gene expression, lines of transgenic mice were generated which contain murine alpha A-crystallin promoter sequences [-111 to +46 (alpha 111), -88 to +46 (alpha 88), and -34 to +46 (alpha 34)] fused to the bacterial chloramphenicol acetyltransferase (CAT) gene and CAT expression was analyzed. Mice carrying the alpha 111-CAT or the alpha 88-CAT fusion transgene expressed CAT exclusively in lens, except for one line containing alpha 111-CAT, which expressed low levels of CAT in several nonlenticular tissues. Transcription from these promoters in lens initiated at the same site as the endogenous alpha A-crystallin promoter. In one line of mice alpha 88-CAT transgene became active in the lens during embryonic development at approximately the same time that the alpha A-crystallin gene normally begins to be expressed. In contrast, the alpha 34-CAT fusion transgene, containing the TATA box but no sequences further upstream, was inactive in transgenic mice. Our data suggest that 134 bp of sequence (-88 to +46) in the murine alpha A-crystallin gene is sufficient to provide lens specificity, although we cannot rule out the possibility that other sequences also contribute to promoter function.
    [Abstract] [Full Text] [Related] [New Search]