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  • Title: [Use of a selective sulfite-reducing medium for the isolation of Clostridium perfringens].
    Author: Konecný S, Mícková V.
    Journal: Vet Med (Praha); 1979 Nov; 24(11):691-7. PubMed ID: 229618.
    Abstract:
    The effect of sulphite-reduction in 33 sample bacterial strains was tested. With regard to the capacity of reducing sulphite in modified sulphite-reduction media in a wide scale of bacterial strains the possibility of an application of selective media with an addition of various concentrations of antibiotic solutions was checked. A concentration of 750 microgram of D-cycloserine per 1 ml of the sulphite-reduction medium appeared to be the most advantageous for the isolation and detection of sulphite-reductive clostridia, above all of Clostridium perfringens. This concentration ensured also a sufficient inhibition of undesirable bacteria without any affection of the growth and capacity of Clostridium perfringens to reduce sulphite in the applied medium.
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