These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Development of a novel europium(III) complex-based luminescence probe for time-resolved luminescence imaging of the nitric oxide production in neuron cells.
    Author: Liu M, Ye Z, Wang G, Yuan J.
    Journal: Talanta; 2012 Sep 15; 99():951-8. PubMed ID: 22967648.
    Abstract:
    Nitric oxide (NO) is a signaling molecule, and plays an important role in some human neuroses. In this work, a novel europium(III) complex, [4'-(3-methylamino-4-aminophenoxy)-2,2':6',2''-terpyridine-6,6''-diyl] bis(methylenenitrilo) tetrakis(acetate)-Eu(3+) (MATTA-Eu(3+)), has been designed and synthesized as a luminescent probe for the time-resolved luminescence detection of NO. The complex itself is weakly luminescent, but can specifically react with NO under the aerobic conditions to form its triazole derivative, [4'-(3-methyl-4-benzotriazol-6-yl-oxy)-2,2':6',2''-terpyridine-6,6''-diyl] bis(methylenenitrilo) tetrakis(acetate)-Eu(3+) (MBTTA-Eu(3+)), accompanied by a remarkable luminescence enhancement with a long luminescence lifetime. The luminescence response of the complex to NO is rapid, highly selective and sensitive, and pH-insensitive in aqueous solutions. These properties enable MATTA-Eu(3+) to be used as a turn-on luminescent probe for the selective recognition and sensitive time-resolved luminescence detection of NO in a wider pH range. The probe-loaded PC12 cells were prepared, and used for the time-resolved luminescence imaging detection of the glutamate-induced NO production in the cells. The result demonstrated the practical utility of the probe for imaging the NO production in neuron cells.
    [Abstract] [Full Text] [Related] [New Search]