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  • Title: Proton transfer in a reaction catalyzed by onion lachrymatory factor synthase.
    Author: Masamura N, Aoyagi M, Tsuge N, Kamoi T, Imai S.
    Journal: Biosci Biotechnol Biochem; 2012; 76(9):1799-801. PubMed ID: 22972339.
    Abstract:
    We produced a single deuterated lachrymatory factor (propanthial S-oxide, m/z = 91) in a model reaction system comprising purified alliinase, lachrymatory factor synthase (LFS), and (E)-(+)-S-(1-propenyl)-L-cysteine sulfoxide ((E)-PRENCSO) in D(2)O. Onion LFS reacted with the degraded products of (E)-PRENCSO by alliinase, but not with those of (Z)-PRENCSO. These findings indicate that onion LFS is an (E)-1-propenylsulfenic acid isomerase.
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