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  • Title: Single-cell gel electrophoresis (Comet) assay in nano-genotoxicology.
    Author: Pacurari M, Castranova V.
    Journal: Methods Mol Biol; 2012; 926():57-67. PubMed ID: 22975956.
    Abstract:
    The Comet assay, or single-cell gel electrophoresis assay, is an easy and simple yet reliable method to evaluate DNA damage in cells. Under the alkaline conditions of this method, DNA strand breaks and alkaline-labile sites are detected. Here we describe the alkaline version of the Comet assay with applications in testing nanoparticles.
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