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  • Title: High molecular weight forms of basic fibroblast growth factor recognized by a new anti-bFGF antibody.
    Author: Grothe C, Zachmann K, Unsicker K, Westermann R.
    Journal: FEBS Lett; 1990 Jan 15; 260(1):35-8. PubMed ID: 2298295.
    Abstract:
    An antibody against basic fibroblasts growth factor (bFGF) was raised using purified bovine pituitary bFGF. Western blot analysis revealed immunoreactive bands at 18, 24, 30-33 and 46 kDa in immunoaffinity purified extracts of pituitary and adrenal gland using this antibody. A similar staining pattern was obtained with ovary extracts with the exception of the missing 18 kDa band. A second anti-bFGF antibody raised against a synthetic peptide comprising the 24 N-terminal amino acids of bFGF reacted with the 18 kDa and the 46 kDa band of immunoaffinity purified ovary and adrenal gland extracts.
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