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  • Title: Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source.
    Author: Liu R, Liang L, Cao W, Wu M, Chen K, Ma J, Jiang M, Wei P, Ouyang P.
    Journal: Bioresour Technol; 2013 May; 135():574-7. PubMed ID: 23010211.
    Abstract:
    Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation of sugarcane bagasse hydrolysate by engineered E. coli BA204, a pflB, ldhA, and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase from Bacillus subtilis 168, produced a final succinate concentration of 15.85 g L(-1) with a high yield of 0.89 g L(-1) total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent anaerobic succinate production, with a final succinate concentration of 18.88 g L(-1) and a yield of 0.96 g g(-1) total sugar after 24 h of anaerobic fermentation. The high succinate yield from sugarcane bagasse hydrolysate demonstrated a great potential application of renewable biomass as a feedstock for the economical production of succinate using metabolically engineered E. coli.
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